Quantile
treatment effects are estimated to study the impacts of household credit access
on health spending by poor households in one District of Ho Chi Minh City,
Vietnam.
There
are significant positive effects of credit on the health budget shares of
households with low health care spending. In contrast, when an average
treatment effect is estimated, there is no discernible impact of credit access
on health spending.
Hence,
typical approaches to studying heterogeneous credit impacts that only consider
between-group differences and not differences over the distribution of outcomes
may miss some heterogeneity of interest to policymakers.
Introduction:
Endothelial
progenitor cells (EPCs) are important progenitor cells in vasculogenesis as
well as in tissue engineering.
However,
few EPCs can be isolated from bone marrow, peripheral blood and umbilical cord
blood.
Moreover,
their in vitro proliferation potential is also limited. Therefore, this study
aimed to produce EPCs from direct reprogramming of fibroblasts by transduction
with certain specific factors.
Methods:
Human
fibroblasts were collected from human skin by published protocols.
The
cells were transduced with 2 viral vectors containing 5 factors, including
Oct3/4, Sox2, Klf4, c-Myc (plasmid 1), and VEGFR2 (plasmid 2). Transduced cells
were treated with endothelial cell medium for 21 days.
The
cells were analyzed for expression of Oct3/4, Sox3, Klf4, c-Myc and VEGFR2 at day
5, and for EPC phenotype at day 21
Results:
The
results showed that after 5 days of transduction, fibroblasts acquired partial
pluripotency.
After
21 days of transduction and culture in endothelial cell medium, the cells
exhibited endothelial markers (e.g. CD31 and VEGFR2) and formed blood
vessel-like capillaries.
Conclusion:
Our findings suggest another strategy for direct reprogramming of fibroblasts
into EPCs.
Title:
| Direct reprogramming of fibroblasts into endothelial progenitor cells by defined factors | |
| Authors: | Truong, MTH Huynh, OT Pham, LH |
| Keywords: | Direct reprogramming Fibroblasts Endothelial progenitor cells |
| Issue Date: | 2016 |
| Publisher: | BIOMEDPRESS, BIOMEDPRESS, HO CHI MINH, 00000, VIETNAM |
| Citation: | ISIKNOWLEDGE |
| Abstract: | Introduction: Endothelial progenitor cells (EPCs) are important progenitor cells in vasculogenesis as well as in tissue engineering. However, few EPCs can be isolated from bone marrow, peripheral blood and umbilical cord blood. Moreover, their in vitro proliferation potential is also limited. Therefore, this study aimed to produce EPCs from direct reprogramming of fibroblasts by transduction with certain specific factors. Methods: Human fibroblasts were collected from human skin by published protocols. The cells were transduced with 2 viral vectors containing 5 factors, including Oct3/4, Sox2, Klf4, c-Myc (plasmid 1), and VEGFR2 (plasmid 2). Transduced cells were treated with endothelial cell medium for 21 days. The cells were analyzed for expression of Oct3/4, Sox3, Klf4, c-Myc and VEGFR2 at day 5, and for EPC phenotype at day 21. Results: The results showed that after 5 days of transduction, fibroblasts acquired partial pluripotency. After 21 days of transduction and culture in endothelial cell medium, the cells exhibited endothelial markers (e.g. CD31 and VEGFR2) and formed blood vessel-like capillaries. Conclusion: Our findings suggest another strategy for direct reprogramming of fibroblasts into EPCs. |
| Description: | BIOMEDICAL RESEARCH AND THERAPY Volume: 3 Issue: 8 Pages: 780-789 ; TNS06514 |
| URI: | http://repository.vnu.edu.vn/handle/VNU_123/29263 |
| Appears in Collections: | Bài báo của ĐHQGHN trong Web of Science |
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