Optimization of medium for the production of a novel aquaculture probiotic, streptomyces sp. a1 using central composite design of response surface methodology
http://repository.vnu.edu.vn/handle/VNU_123/32560
A strain, Streptomyces sp. A1 isolated from shrimp pond sediments has been identified as an aquaculture probiotic antagonistic to pathogenic Vibrio harveyi V7.
In the present study, the different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity, following response surface methodology (RSM).
Accordingly, the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design CCD of RSM for the final optimization. The response surface plots of biomass production showed similar pattern with that of antagonistic activity, which indicated a correlation between the biomass and antagonism.
The optimum concentrations of the carbon and nitrogen source, and growth factors for both biomass and antagonistic activity were starch (14.62 g/1), casein (0.93 g/1), NaCl (15.6 g/1), DL-α-alanine (0.233 g/1), and vitamin B6 (0.023 g/1).
A strain, Streptomyces sp. A1 isolated from shrimp pond sediments has been identified as an aquaculture probiotic antagonistic to pathogenic Vibrio harveyi V7.
In the present study, the different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity, following response surface methodology (RSM).
Accordingly, the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design CCD of RSM for the final optimization. The response surface plots of biomass production showed similar pattern with that of antagonistic activity, which indicated a correlation between the biomass and antagonism.
The optimum concentrations of the carbon and nitrogen source, and growth factors for both biomass and antagonistic activity were starch (14.62 g/1), casein (0.93 g/1), NaCl (15.6 g/1), DL-α-alanine (0.233 g/1), and vitamin B6 (0.023 g/1).
| Title: | Optimization of medium for the production of a novel aquaculture probiotic, streptomyces sp. a1 using central composite design of response surface methodology |
| Authors: | Chau, Ngo Thi Tuong Masaru, MATSUMOTO Ikuo, MIYAJIMA 2 |
| Keywords: | Aquaculture probiotic Central composite design Response surface methodology Streptomyces sp. A1 Vibrio harveyi V7 |
| Issue Date: | 2014 |
| Publisher: | Journal of the Faculty of Agriculture, Kyushu University |
| Citation: | Scopus |
| Abstract: | A strain, Streptomyces sp. A1 isolated from shrimp pond sediments has been identified as an aquaculture probiotic antagonistic to pathogenic Vibrio harveyi V7. In the present study, the different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity, following response surface methodology (RSM). Accordingly, the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design CCD of RSM for the final optimization. The response surface plots of biomass production showed similar pattern with that of antagonistic activity, which indicated a correlation between the biomass and antagonism. The optimum concentrations of the carbon and nitrogen source, and growth factors for both biomass and antagonistic activity were starch (14.62 g/1), casein (0.93 g/1), NaCl (15.6 g/1), DL-α-alanine (0.233 g/1), and vitamin B6 (0.023 g/1). |
| Description: | Journal of the Faculty of Agriculture, Kyushu University, Volume 59, Issue 1, February 2014, Pages 25-32 Journal of the Faculty of Agriculture, Kyushu University |
| URI: | http://repository.vnu.edu.vn/handle/VNU_123/32560 |
| ISSN: | 00236152 |
| Appears in Collections: | Bài báo của ĐHQGHN trong Scopus |
Nhận xét
Đăng nhận xét